Theorganism is rarely reported to occur in clinical specimens. During a period of seven years 123 strains, both pigmented and nonpigmented varieties were isolated. Serretia rubidaea for biofilm development capability jabun naher1, shahidul a. Production of extracellular lipase in submerged culture of serratia rubidaea has been investigated. Files are available under licenses specified on their description page. The only serratia species recognized in the 8th edition of bergeys manual was serratia marcescens 1. Based on 16s rrna gene sequence analysis, the isolate was identified as serratia marcescens. Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent. Abstract microorganisms are known to produce a variety of pigments. Investigation of lipase production by milk isolate serratia rubidaea. A study was made of the biochemical reactions given by 109 cultures of enterobacter liquefaciens and 49 strains of serratia rubidaea. The most common and pathogenic of the species in the genus, s. Since 1823, 15 species have been identified mahlen, 2011 but the. Genero enterobacter y serratia infeccion salud publica.
Serratia is a genus of gramnegative, facultatively anaerobic, rodshaped bacteria of the family yersiniaceae. Alternatively, you can download the file locally and open with any standalone pdf reader. This species was first described as bacterium rubidaea in 1940 and presently recognized as s. Isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits. The full text of this article is available in pdf format. Serratia species, serratia marcescens, serratia liquefaciens, serratia plymuthica, serratia rubidaea, serratia fonticola, serratia marnorubra, serratia proteamaculans, and serratia odorifera, are opportunistic pathogens and can be isolated from water, soil, plants, and air. Apart from serratia marcescens and the serratia liquefaciens complex s. Supporting information the short term effect of the soil. Sustainable biosurfactant produced by serratia marcescens ucp. However, symptoms depend on the infection site and disease. Studies on the response of microbes to environmental stressors form a thrust area of contemporary. Serratia rubidaea as an invasive pathogen article pdf available in journal of clinical microbiology 341. This is the first case in which the organism wasfound to be responsible for invasive humaninfection.
Serratia rubidaea bacteremia request pdf researchgate. Taxonomically, the genus serratia is confusing, and currently there are 14 recognized species, with 2 subspecies, in the genus table 1. The lipase production was optimized in shake flask experiments. Escherichia, klebsiella, enterobacter, citrobacter, serratia presence of coliforms in water is indicate of impure water and of poor sewage treatment i. A serratia rubidaea isolate with wetting activity when grown at 30 but not 37 degrees c was examined for the production of specific lipids. Aug 15, 2019 biochemical test and identification of serratia marcescens. Serratia marcescens is the primary pathogenic species of serratia. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. Brenner established serratia rubidaea as a later homotypic synonym of serratia marinorubra zobell and upham 1944 approved lists 1980 in 1984. Serratia species secrete several virulence factors, such as dnase, lipase, gelatinase, hemolysin, proteases, chitinase. The full text of this article is available as a pdf 157k. Serratia is a short, facultatively anaerobic, gramnegative, rodshaped bacteria of the enterobacteriaceae family. Use of bileesculin agar for rapid differentiation of.
Antagonistic activity of bacillus amyloliquefaciens subsp. The clinical significance of the organism was undetermined due to its apparent eradication from this location by therapy with topical 1% silver sulfadiazine. One preserved isolated subculture was identified on the basis of their morphological, cultural and biochemical characteristics. Oxidase negative gram negative rods enterobacteriaece. Ammonia produced by bacterial colonies promotes growth of. Klebsiella pneumoniae, enterobacter aerogenes, serratia marcescens, and serratia rubidaea produced a positive reaction in 4 h. Highest tensioactive activity detected in current study in flasks with agitation can be linked to the physiological function of the biosurfactants. Microbial pigments find several applications and are thus of commercial importance. The present study demonstrates the plant growth promoting pgp potential of a bacterial isolate cdp isolated from capparis decidua plant, and its ability to protect plants from the deleterious effect of biotic and abiotic stressors. Studies on the antagonistic effect of serretia rubidaea. Outbreaks of serratia marcescens and serratia rubidaea bacteremia. Analysis of the genomic sequences and metabolites of serratia. The species serratia rubidaea was originally described by ewing et al.
In their paper about the taxonomy of serratia, grimont and coworkers 2 described four species within the genus serratia. Serratia rubidaea an aerobic gramnegative bacillus that grows readily on most microbiological media producing a red prodigiosin colony. Isolation and characterization of serratia rubidaea from dark. Effects of bacterial growth and gas phase ammonia on medium ph and serratia rubidaea growth. Serratia species are opportunistic gramnegative bacteria classified in the tribe klebsielleae and the large family enterobacteriaceae. From naturally infected tomato fruits showing dark brown irregularly shaped spots, 36 bacterial isolates were recovered and identified on phenotypic characteristics and sequences of the gene encoding the 16s rrna. S7 1 ec council decision of 19 december 2002, establishing criteria and procedures for the acceptance of waste at landfills. Serratia rubidaea definition of serratia rubidaea by. The observed ph and temperature range optimum for maximum lipase production were 78 and 3040 c, respectively. Biblioteca italiana o sia giornale di letteratura, scienze e arti anno viii 30. Pdf isolation and characterization of serratia rubidaea. Natural antimicrobial susceptibilities of strains of unusual serratia. Among the pgp traits, the isolate was found to be positive for. Serratia species, serratia marcescens, serratia liquefaciens, serratia.
The other remaining eight genera exhibited varying results. Using serratia plymuthica to control fungal pathogens of plants. A total of 167serratia strains was isolated from these plant samples and identified to species and biogroups. The isolate was belonged to the genus serretia and provisionally identified as. Staphylococcus warnerii, i pseudomonas aurantiaca, j serratia rubidaea, and one yeast species, k pichia anomalia. Only serratia marcescens has been established as a.
When it enters the bloodstream, endotoxins are released that cause fever, septic shock, thrombocytopenia and disseminated intravascular coagulation. Nowadays, recognized species within the genus serratia are s. Voc emission of various serratia species and isolates and. Pdf serratias are widely distributed in soil, air and water, plantand animalassociated bacteria. If the species was not widely studied, the american type culture collection atcc was used to determine the optimal ph for storage. Pdf isolation and characterization of serratia rubidaea from dark. Serratia is a ubiquitous opportunistic pathogen that is frequently present under damp conditions in food, plants, animals, soil, and household items e. Using serratia plymuthica to control fungal pathogens of. Introduction the genus serratia rubidaea is gramnegative speculator pathogens, known to survive harsh environmental surroundings and is a leading cause of hospital acquired infections. Interest in biological control of plant pathogens has increased in recent years fuelled by trends in agriculture. Pdf investigation of lipase production by milk isolate.
Isolation and characterization of serratia rubidaea from. Serratia rubidaea synonyms, serratia rubidaea pronunciation, serratia rubidaea translation, english dictionary definition of serratia rubidaea. Bergeys manual of determinative bacteriology all of the unknowns will fall into the following groups in bergeys manual of determinative bacteriology the pink book on the shelf in the laboratory. Biochemical characterization of serratia liquefaciens and. Using the selective caprylatethallous agar medium, the presence ofserratia species was systematically examined in 623 plant samples. Serratia is a genus of gramnegative, facultatively anaerobic, rodshaped bacteria of the family. The saprophytic bacterium serratia plymuthica was recovered from a facial wound burn site of a pediatric patient. Biblioteca italiana o sia giornale di letteratura, scienze e. Mar 08, 2011 isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits abdalla, mohamed. If the species was not widely studied, the american type culture collection atcc was used to.
Page 4 flow chart gram negative reaction possibilities eliminated pseudomonas enterobacteriaceae neisseria veillonella bacillus mycobacterium lactobacillus, listeria kurthia micrococcus, planococcus, sporosarcina staphylococcus streptococcus enterococcus morphology rods possibilities eliminated pseudomonas enterobacteriaceae neisseria veillonella. Members of the genus serratia, particularly the type species serratia marcescens, cause important infections in humans, animals, and insects. Part of the microgen bioproducts ltd, unit 1, watchmoor point, camberley, surrey gu15 3ad, united kingdom t. Sustainable biosurfactant produced by serratia marcescens. It was discovered in 1819 by bartolomeo bizio in padua, italy. Five isolates showing spots on tomato fruits in the pathogenicity test with healthy tomato fruits belong to the. Khan2 1department of microbiology, university of chittagong, chittagong, bangladesh 2institute of radiation and polymer technology, bangladesh atomic energy commission, dhaka, bangladesh. Serratia rubidaea is an opportunist pathogenic bacterium, it is rarely identified in man.
Flow chart page 4 flow chart gram negative reaction. Mar 19, 2018 this page was last edited on 19 march 2018, at. Paraplegia could be a predisposing factor for this organism to cause urinary tract infection. Proizvodnja lipaze s pomocu bakterije serratia rubidaea. Maldi biotyper smart ca system the highend option with increased sample throughput the maldi biotyper ca system is brukers pioneering solution for rapid and accurate microbial. The infection was cleared after adequate treatment with antibiotics. The most commonly known humanderived species is serratia marcescens.
Bacterial contamination of fresh tomato fruits is of great concern. They have created significant clinical challenges for clinicians because they are not consistently identified by routine screening methods and are. Isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits article pdf available in phytoparasitica 392. The use of this medium in conjunction with triple sugar ironlysine iron agar has been of great value in differentiating the klebsiellaenterobacter serratia. Jun 20, 2016 the observed increase in plant growth by acc deaminaseproducing serratia marcecens cdp is also supported by other observations, where acc deaminaseproducing bacteria were shown to enhance plant growth by lowering the ethylene level 58, 59. The reduction in surface tension is commonly used as a primary criterion for selecting biosurfactantproducing microorganisms 2, 27, 28. Serratia odorifera biogroup 1 wasisolated fromthe blood andurine ofanalcoholic malewithcirrhosis and signs ofseptic shock. It is capable of growth between 4oc and 37oc with some isolates capable of growth at 40oc. Voluntary surveillance of bacteraemia due to klebsiella spp.
Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. Isolation of serratia plymuthica from a human burn site. All structured data from the file and property namespaces is available under the creative commons cc0 license. Choose from 80 different sets of serratia flashcards on quizlet. Two novel lipids rubiwettins r1 and rg1 were isolated and shown to be able to lower the surface tension of saline to 26 mnm. Uniform and characteristicserratia populations were found in figs and coconuts. Biochemical characteristics, antimicrobial sensitivity and clinical significance sd puthucheary mbbs, dipbactc and yf ngeow mbbs, mscc summary serratia marcescens has become an important nosocomial pathogen. Serratia rubidaea definition of serratia rubidaea by the. Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center.
This name appeared on the approved lists of bacterial names 1980. They appear ubiquitously inon soil, water, plants and animals, including humans. In this study, surface tension was used as response variable, and the effects of independent variables lactose, cww and co concentrations as well as the interactions of them, were analyzed by estimated effects represented in table 3 and fig. Nag agar with phenol red was used to visualize ph changes. Voluntary surveillance of bacteraemia klebsiella spp. Although several recent studies disclosed a number of interesting biological properties of prodigiosin, this paper reports a higher value of prodigiosin production by a newly isolated indigenous marine bacteria serratia rubidaea rather than serratia marcescens from a relatively cheap medium.
Serratia serashea, a genus of motile, peritrichous. The genus serratia is composed of facultative anaerobic, rodshaped gammaproteobacteria with peritrichous flagella summarized in mahlen, 2011. The taxonomy of the other currently recognized serratia species is clearer. Serratia are widespread in the environment, but are not a common component of the human fecal flora. Urinary tract infections are asymptomatic for 3050% of infected individuals, with the rest. Serratia rubidaea is a less welldescribed member of the genus and is chiefly found in soil, water and food. Pdf role of pigment in the stress response of serratia. For example, patients with serratia sepsis may present with fever, chills, shock, and respiratory. Request pdf serratia rubidaea bacteremia serratia rubidaea is an opportunist. Serratia species isolated from plants springerlink. Investigation of lipase production by milk isolate. Pursuant to article 16 of annex ii to directive 199931ec. The second organism, serratia marcescens, might be present in the vomit due to the same contaminated food.
Carotenoids are effective antioxidants and are widely used as food colorants. Surfaceactive novel glycolipid and linked 3hydroxy fatty. This study was performed to compare the growth dynamics of serretia in single and coculture biofilms condition and also evaluated its antagonistic effect against pathogenic bacteria. Serratia rubidaea motile, red pigment enterobacter aerogenes motile, no pigment. Hosted by the usgs core science analytics and synthesis.
Serratia marcescens after initial outbreaks in the northeastern u. They are gram ve, catalase positive, oxidase negative, mr negative and vp positive bacteria. Page designed through the cooperative efforts of interagency itis teams. Lettera di bartolomeo bizio al chiarissimo canonico angelo bellani sopra il fenomeno della polenta porporina. Serratia rubidaea is an opportunist pathogenic bacterium, it is rarely identified in man, and when so, generally found in the respiratory tract, wounds, feces, bile, but also in blood.
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